Preparation of lactose hydrolysed milk using β-galactosidase enzyme extracted from potential Lactobacillus cultures
نویسندگان
چکیده
منابع مشابه
in-house production of lactose-hydrolysed milk by beta-galactosidase from lactobacillus bulgaricus
crude enzyme (beta-galactosidase) extract (cee) was produced by lactobacillus ssp. bulgaricus chr hansen lb-12 and was applied in sterile milk which had been processed through ultra high temperature method (uht milk), for hydrolyzing lactose. lactosehydrolyzed milk was also produced by a pure and commercially available betagalactosidase (maxilact). optimum quantities of cee and maxilact enz...
متن کاملGalacto-oligosaccharide synthesis from lactose solution or skim milk using the β-galactosidase from Bacillus circulans.
The synthesis of galacto-oligosaccharides (GOS) catalyzed by a novel commercial preparation of β-galactosidase from Bacillus circulans (Biolactase) was studied, and the products were characterized by MS and NMR. Using 400 g/L lactose and 1.5 enzyme units per milliliter, the maximum GOS yield, measured by HPAEC-PAD analysis, was 165 g/L (41% w/w of total carbohydrates in the mixture). The majo...
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In some people the inadequate amount of Beta-galactosidase enzyme, in layer of villous epithelium of the small intestine, causes the disorder of lactose intolerance. This examination was done to observe the existence of Beta-galactosidase enzyme that is produced by separated Lactobacillus from samples of dairy products (milk and cheese). Materials and Methods: Pasteurized and local...
متن کاملPreparation of lactose-free pasteurized milk with a recombinant thermostable β-glucosidase from Pyrococcus furiosus
BACKGROUND Lactose intolerance is a common health concern causing gastrointestinal symptoms and avoidance of dairy products by afflicted individuals. Since milk is a primary source of calcium and vitamin D, lactose intolerant individuals often obtain insufficient amounts of these nutrients which may lead to adverse health outcomes. Production of lactose-free milk can provide a solution to this ...
متن کاملBioaffinity Based Immobilization of Almond (Amygdalus communis) b-galactosidase on Con A-layered Calcium Alginate-cellulose Beads: Its Application in Lactose Hydrolysis in Batch and Continuous Mode
In this study, immobilization of partially purified almond (Amygdalus communis) β-galactosidase on Con A layered calcium alginate-cellulose beads was investigated. Immobilized β-galactosidase retained 72% of theinitial activity after crosslinking by glutaraldehyde. Both soluble and immobilized enzyme exhibited the samepH and temperature optima at pH 5.5 and 50ºC, respectively. Howev...
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ژورنال
عنوان ژورنال: Vol 72, No 1 (2019)
سال: 2019
ISSN: 2454-2172
DOI: 10.33785/ijds.2019.v72i01.009